Human Fc gamma RIIIA/CD16a (V176) Protein Recombinant
Category: Receptors$162.00 – $540.00
Description
Accession
AAH17865
Source
DNA sequence encoding Human Fc gamma RIIIA/CD16a (V176) including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Fc gamma RIIIA/CD16a (V176) is a protein consisting of amino acid residues [Gly17-Gln208] ,The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fc gamma RIIIA/CD16a (V176) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human Fc gamma RIIIA/CD16a (V176) Protein Tris-Bis PAGE
- Human Fc gamma RIIIA (V176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Fc gamma RIIIA/CD16a (V176) Protein SEC-HPLC
- The purity of Fc gamma RIIIA (V176) is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIIA/CD16a (V176) Protein ELISA Data
- Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA.
Human Fc gamma RIIIA/CD16a (V176) Protein SPR Data
- Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).
