Description
Accession
P31994
Source
DNA sequence encoding Human Fc gamma RIIB/CD32b including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Fc gamma RIIB/CD32b is a protein consisting of amino acid residues [Ala46-Pro217] ,The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fc gamma RIIB/CD32b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Methods
Human Fc gamma RIIB/CD32b Protein Tris-Bis PAGE
- Human Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Fc gamma RIIB/CD32b Protein SEC-HPLC
- The purity of Human Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.
Human Fc gamma RIIB/CD32b Protein SPR Data
- Human Fc gamma RIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 9.4μM as determined in SPR assay (Biacore T200).
