Human Fc epsilon RI alpha/FCER1a Protein Recombinant
Category: Receptors$137.00 – $696.00
Description
Accession
P12319
Source
DNA sequence encoding Human Fc epsilon RI alpha/FCER1a including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Fc epsilon RI alpha/FCER1a is a protein consisting of amino acid residues [Val26-Gln205] ,The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fc epsilon RI alpha/FCER1a was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Molecular function
Methods
Human Fc epsilon RI alpha/FCER1a Protein Tris-Bis PAGE
- Human Fc epsilon RI alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Fc epsilon RI alpha/FCER1a Protein SEC-HPLC
- The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.
Human Fc epsilon RI alpha/FCER1a Protein ELISA Data
- Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA.
