Description
Accession
P25445
Source
DNA sequence encoding Human Fas/TNFRSF6/CD95 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Fas/TNFRSF6/CD95 is a protein consisting of amino acid residues [Gln26-Asn173] ,The protein has a predicted MW of 13.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fas/TNFRSF6/CD95 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P06239
Interactor
P07947
Interactor
P19174
Interactor
Interactor
P06239
Interactor
P12931
Interactor
P19174
Interactor
Interactor
Q9UER7
Interactor
Interactor
Q03135
Interactor
Q13158
Interactor
Q13546
Interactor
Molecular function
Molecular function
Methods
Human Fas/TNFRSF6/CD95 Protein Tris-Bis PAGE
- Human Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Fas/TNFRSF6/CD95 Protein SPR Data
- Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
