Description
Accession
P29323
Source
DNA sequence encoding Human EPHB2 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human EPHB2 is a protein consisting of amino acid residues [Val19-Leu543] ,The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EPHB2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human EPHB2 Protein Tris-Bis PAGE
- Human EPHB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human EPHB2 Protein SEC-HPLC
- The purity of Human EPHB2 is greater than 95% as determined by SEC-HPLC.
Human EPHB2 Protein ELISA Data
- Immobilized Human EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA.
