Description
Accession
P01133
Source
DNA sequence encoding Human EGF including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human EGF is a protein consisting of amino acid residues [Asn971-Arg1023] ,The protein has a predicted MW of 5.6 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGF was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human EGF Protein Tris-Bis PAGE
- Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human EGF Protein SEC-HPLC
- The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Human EGF Protein ELISA Data
- Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA.
Human EGF Protein SPR Data
- Human EGFR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human EGF, No Tag with an affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
