Description
Accession
P01133
Source
DNA sequence encoding Human EGF including a N-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human EGF is a protein consisting of amino acid residues [Asn971-Arg1023] ,The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human EGF was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human EGF Protein
- Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human EGF Protein
- The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
Human EGF Protein SPR Data
- Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.16 nM as determined in SPR assay (Biacore T200).
