Description
Accession
Q15762
Source
DNA sequence encoding Human DNAM-1/CD226 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human DNAM-1/CD226 is a protein consisting of amino acid residues [Glu19-Asn247] ,The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human DNAM-1/CD226 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Human DNAM-1/CD226 Protein Tris-Bis PAGE
- Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human DNAM-1/CD226 Protein SEC-HPLC
- The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
Human DNAM-1/CD226 Protein ELISA Data
- Immobilized Human DNAM-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.43μg/ml determined by ELISA.
Human DNAM-1/CD226 Protein SPR Data
- Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay (Biacore T200).
