Description
Accession
Q9NYJ7
Source
DNA sequence encoding Human DLL3 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human DLL3 is a protein consisting of amino acid residues [Ala27-Arg490] ,The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human DLL3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human DLL3 Protein Tris-Bis PAGE
- Human DLL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human DLL3 Protein SEC-HPLC
- The purity of Human DLL3 is greater than 95% as determined by SEC-HPLC.
Human DLL3 Protein ELISA Data
- Immobilized Human DLL3, His Tag at 0.1μg/mL (100μl/well). Dose response curve for Anti- DLL3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
Human DLL3 Protein Batch to Batch Consistency
- Immobilized Human DLL3, His Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 5.0ng/ml / 4.5ng/ml / 5.0ng/ml determined by ELISA.
