Description
Accession
Q08345
Source
DNA sequence encoding Human DDR1 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human DDR1 is a protein consisting of amino acid residues [Asp21-Ala417] ,The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human DDR1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P02461
Biological Process
Biological Process
Molecular function
Molecular function
Methods
Human DDR1 Protein Tris-Bis PAGE
- Human DDR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human DDR1 Protein SEC-HPLC
- The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC.
Human DDR1 Protein ELISA Data
- Immobilized Human DDR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.
Human DDR1 Protein SPR Data
- Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).
