Human/Cynomolgus/Rhesus macaque CD28 Protein Recombinant
Category: Receptors$202.00 – $675.00
Description
Accession
P10747
Source
DNA sequence encoding Human/Cynomolgus/Rhesus macaque CD28 including a N-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human/Cynomolgus/Rhesus macaque CD28 is a protein consisting of amino acid residues [Asn19-Pro152] ,The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human/Cynomolgus/Rhesus macaque CD28 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P0A0L2
Interactor
P01552
Interactor
Interactor
Methods
Human/Cynomolgus/Rhesus macaque CD28 Protein Tris-Bis PAGE
- Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human/Cynomolgus/Rhesus macaque CD28 Protein SEC-HPLC
- The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.
Human/Cynomolgus/Rhesus macaque CD28 Protein ELISA Data
- Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.
Human/Cynomolgus/Rhesus macaque CD28 Protein SPR Data
- Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).
