Description
Accession
NP_001807
Source
DNA sequence encoding Human CEACAM-8/CD66b including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CEACAM-8/CD66b is a protein consisting of amino acid residues [Gln35-Ser319] ,The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CEACAM-8/CD66b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human CEACAM-8/CD66b Protein Tris-Bis PAGE
- Human CEACAM-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CEACAM-8/CD66b Protein SEC-HPLC
- The purity of Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.
Human CEACAM-8/CD66b Protein SPR Data
- Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 4.59 μM as determined in SPR assay (Biacore T200).
