Description
Accession
Q8N4D0
Source
DNA sequence encoding Human CEACAM-5/CD66e including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CEACAM-5/CD66e is a protein consisting of amino acid residues [Lys35-Ala685] ,The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CEACAM-5/CD66e was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human CEACAM-5/CD66e Protein Tris-Bis PAGE
- Human CEACAM-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CEACAM-5/CD66e Protein SEC-HPLC
- The purity of Human CEACAM-5 is greater than 95% as determined by SEC-HPLC.
Human CEACAM-5/CD66e Protein ELISA Data
- Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.
Human CEACAM-5/CD66e Protein SPR Data
- Human CEACAM-5, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CEACAM5 Antibody, hFc Tag with an affinity constant of 0.12nM as determined in SPR assay (Biacore T200).
