Description
Accession
Q9H5V8
Source
DNA sequence encoding Human CDCP1 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CDCP1 is a protein consisting of amino acid residues [Phe30-Leu666] ,The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CDCP1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P06241
Interactor
P12931
Interactor
P07947
Methods
Human CDCP1 Protein Tris-Bis PAGE
- Human CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CDCP1 Protein SEC-HPLC
- The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC.
Human CDCP1 Protein ELISA Data
- Immobilized Human CDCP1 at 2μg/ml (100μl/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Human CDCP1 Protein SPR Data
- Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200).
