Description
Accession
P01732
Source
DNA sequence encoding Human CD8 alpha/CD8A including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD8 alpha/CD8A is a protein consisting of amino acid residues [Ser22-Asp182] ,The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD8 alpha/CD8A was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human CD8 alpha/CD8A Protein Tris-Bis PAGE
- Human CD8 alpha/CD8A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD8 alpha/CD8A Protein SEC-HPLC
- The purity of Human CD8 alpha/CD8A is greater than 95% as determined by SEC-HPLC.
Human CD8 alpha/CD8A Protein ELISA Data
- Immobilized Human CD8 alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 26.3ng/ml determined by ELISA.
