Description
Accession
P21589
Source
DNA sequence encoding Human CD73/NT5E including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD73/NT5E is a protein consisting of amino acid residues [Trp27-Lys547] ,The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD73/NT5E was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human CD73/NT5E Protein Tris-Bis PAGE
- Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD73/NT5E Protein SEC-HPLC
- The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.
Human CD73/NT5E Protein ELISA Data
- Immobilized Human CD73, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA.
