Description
Accession
P06127
Source
DNA sequence encoding Human CD5 including a C-hFc-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD5 is a protein consisting of amino acid residues [Arg25-Asn371] ,The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q00839
Interactor
Q8NEB9
Interactor
P06239
Methods
Human CD5 Protein Tris-Bis PAGE
- Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD5 Protein SEC-HPLC
- The purity of Human CD5 is greater than 95% as determined by SEC-HPLC.
Human CD5 Protein ELISA Data
- Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 19.6ng/ml determined by ELISA.
