Description
Accession
Q08722
Source
DNA sequence encoding Human CD47 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD47 is a protein consisting of amino acid residues [Gln19-Pro139] ,The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD47 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human CD47 Protein Tris-Bis PAGE
- Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD47 Protein SEC-HPLC
- The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
Human CD47 Protein ELISA Data
- Immobilized Human SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 55.6ng/ml determined by ELISA.
Human CD47 Protein ELISA Data
- Immobilized Human SIRP alpha V8, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA.
Human CD47 Protein SPR Data
- Human CD47, hFc Tag captured on Protein A chip via Anti-His Antibody can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
