Description
Accession
Q08722
Source
DNA sequence encoding Human CD47 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD47 is a protein consisting of amino acid residues [Gln19-Pro139] ,The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD47 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human CD47 Protein Tris-Bis PAGE
- Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD47 Protein SEC-HPLC
- The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
Human CD47 Protein ELISA Data
- Immobilized Human CD47, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 28.9ng/ml determined by ELISA.
Human CD47 Protein SPR Data
- Human CD47, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human Sirpa, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).
