Human CD40 Ligand/TNFSF5 Trimer Protein Recombinant
Category: Receptors$193.00 – $641.00
Description
Accession
P29965
Source
DNA sequence encoding Human CD40 Ligand/TNFSF5 Trimer including a N-His-Flag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD40 Ligand/TNFSF5 Trimer is a protein consisting of amino acid residues [Met113-Leu261] ,The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD40 Ligand/TNFSF5 Trimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human CD40 Ligand/TNFSF5 Trimer Protein Tris-Bis PAGE
- Human CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD40 Ligand/TNFSF5 Trimer Protein SEC-HPLC
- The purity of Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Human CD40 Ligand/TNFSF5 Trimer Protein ELISA Data
- Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA.
