Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
P07766
Source
DNA sequence encoding Human CD3E&CD3D/CD3 epsilon&CD3 delta including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta is a protein consisting of amino acid residues [Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 35-42 kDa and 25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein Tris-Bis PAGE
- Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein SEC-HPLC
- The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.
Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein ELISA Data
- Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA.
Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein ELISA Data
- Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA.
Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein SPR Data
- Human CD3E&CD3D, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).
