Description
Accession
P28907
Source
DNA sequence encoding Human CD38 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD38 is a protein consisting of amino acid residues [Val43-Ile300] ,The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD38 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Molecular function
Methods
Human CD38 Protein Tris-Bis PAGE
- Human CD38 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD38 Protein SEC-HPLC
- The purity of Human CD38 is greater than 95% as determined by SEC-HPLC.
Human CD38 Protein ELISA Data
- Immobilized Human CD38, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 28ng/ml determined by ELISA.
Human CD38 Protein SPR Data
- Anti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23μM as determined in a SPR assay (Biacore T200).
