Description
Accession
P32971
Source
DNA sequence encoding Human CD30 Ligand/TNFSF8 including a N-mFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD30 Ligand/TNFSF8 is a protein consisting of amino acid residues [Gln63-Asp234] ,The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD30 Ligand/TNFSF8 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
Methods
Human CD30 Ligand/TNFSF8 Protein Tris-Bis PAGE
- Human CD30 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD30 Ligand/TNFSF8 Protein SEC-HPLC
- The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
Human CD30 Ligand/TNFSF8 Protein ELISA Data
- Immobilized Human CD30, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Human CD30 Ligand, mFc (IgG2a) Tag with the EC50 of 31.9ng/ml determined by ELISA.
