Human CD27 Ligand/CD70 Trimer Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
P32970
Source
DNA sequence encoding Human CD27 Ligand/CD70 Trimer including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD27 Ligand/CD70 Trimer is a protein consisting of amino acid residues [Leu50-Pro193] ,The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD27 Ligand/CD70 Trimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human CD27 Ligand/CD70 Trimer Protein Tris-Bis PAGE
- Human CD27 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD27 Ligand/CD70 Trimer Protein SEC-HPLC
- The purity of Human CD27 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Human CD27 Ligand/CD70 Trimer Protein ELISA Data
- Immobilized Human CD27 Ligand (Trimer) at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 of 63.8ng/ml determined by ELISA.
