Description
Accession
P25063
Source
DNA sequence encoding Human CD24 including a N-GST tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD24 is a protein consisting of amino acid residues [Ser27-Gly59] ,The protein has a predicted MW of 31.6 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD24 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human CD24 Protein Tris-Bis PAGE
- Human CD24 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human CD24 Protein ELISA Data
- Immobilized Human CD24, GST Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 17.7ng/ml determined by ELISA.
