Description
Accession
Q9NNX6
Source
DNA sequence encoding Human CD209/DC-SIGN including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD209/DC-SIGN is a protein consisting of amino acid residues [Gln59-Ala404] ,The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD209/DC-SIGN was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Biological Process
Molecular function
Methods
Human CD209/DC-SIGN Protein Tris-Bis PAGE
- Human CD209 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD209/DC-SIGN Protein SEC-HPLC
- The purity of Human CD209 is greater than 95% as determined by SEC-HPLC.
Human CD209/DC-SIGN Protein SPR Data
- Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200).
