Description
Accession
P15391
Source
DNA sequence encoding Human CD19 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CD19 is a protein consisting of amino acid residues [Pro20-Lys291] ,The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD19 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human CD19 Protein Tris-Bis PAGE
- Human CD19 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CD19 Protein ELISA Data
- Immobilized Human CD19, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA.
Human CD19 Protein ELISA Data
- Immobilized Human CD19, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
