Description
Accession
P13501
Source
DNA sequence encoding Human CCL5 including a N-His,SUMO tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human CCL5 is a protein consisting of amino acid residues [Ser24-Ser91] ,The protein has a predicted MW of 21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CCL5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
P15172
Interactor
P32246
Interactor
P51681
Interactor
P32302
Interactor
P49682
Interactor
P51677
Biological Process
Biological Process
Molecular function
Methods
Human CCL5 Protein Tris-Bis PAGE
- Human CCL5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human CCL5 Protein ELISA Data
- Immobilized Human CCL5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 8ng/ml determined by ELISA.
