Human c-MPL/Thrombopoietin R Protein Recombinant
Category: Receptors$232.00 – $776.00
Description
Accession
P40238
Source
DNA sequence encoding Human c-MPL/Thrombopoietin R including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human c-MPL/Thrombopoietin R is a protein consisting of amino acid residues [Gln26-Trp491] ,The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human c-MPL/Thrombopoietin R was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Methods
Human c-MPL/Thrombopoietin R Protein Tris-Bis PAGE
- Human c-MPL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human c-MPL/Thrombopoietin R Protein SEC-HPLC
- The purity of Human c-MPL is greater than 95% as determined by SEC-HPLC.
Human c-MPL/Thrombopoietin R Protein ELISA Data
- Immobilized Human c-MPL, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.
