Description
Accession
Q86Z14
Source
DNA sequence encoding Human Beta Klotho including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Beta Klotho is a protein consisting of amino acid residues [Met30-Thr983] ,The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Beta Klotho was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human Beta Klotho Protein Tris-Bis PAGE
- Human Beta Klotho on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human Beta Klotho Protein SEC-HPLC
- The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.
Human Beta Klotho Protein ELISA Data
- Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA.
Human Beta Klotho Protein
- Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 18.9ng/ml determined by ELISA.
Human Beta Klotho Protein ELISA Data
- Immobilized Human FGF21, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA.
Human Beta Klotho Protein SPR Data
- Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
