Description
Accession
Q96RJ3
Source
DNA sequence encoding Human BAFFR/TNFRSF13C including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human BAFFR/TNFRSF13C is a protein consisting of amino acid residues [Ser7-Ala71] ,The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human BAFFR/TNFRSF13C was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Human BAFFR/TNFRSF13C Protein Tris-Bis PAGE
- Human BAFFR on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human BAFFR/TNFRSF13C Protein ELISA Data
- Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Human BAFFR/TNFRSF13C Protein ELISA Data
- Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA.
Human BAFFR/TNFRSF13C Protein SPR Data
- Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
