Human BAFF/TNFSF13B/CD257 Trimer Protein Recombinant
Category: Receptors$145.00 – $486.00
Description
Accession
Q9Y275
Source
DNA sequence encoding Human BAFF/TNFSF13B/CD257 Trimer including a N-monomeric hFc,N-Flag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human BAFF/TNFSF13B/CD257 Trimer is a protein consisting of amino acid residues [Thr141-Leu285] ,The protein has a predicted MW of 74.9 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human BAFF/TNFSF13B/CD257 Trimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Molecular function
Methods
Human BAFF/TNFSF13B/CD257 Trimer Protein Tris-Bis PAGE
- Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human BAFF/TNFSF13B/CD257 Trimer Protein SEC-HPLC
- The purity of Human BAFF (Trimer) is greater than 94% as determined by SEC-HPLC.
Human BAFF/TNFSF13B/CD257 Trimer Protein ELISA Data
- Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA.
Human BAFF/TNFSF13B/CD257 Trimer Protein SPR Data
- Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200).
