Human B7-H7/HHLA2 Protein Recombinant
Category: MHC peptide complexes$193.00 – $641.00
Description
Accession
Q9UM44
Source
DNA sequence encoding Human B7-H7/HHLA2 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human B7-H7/HHLA2 is a protein consisting of amino acid residues [Ile23-Asn344] ,The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-H7/HHLA2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human B7-H7/HHLA2 Protein Tris-Bis PAGE
- Human B7-H7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human B7-H7/HHLA2 Protein SEC-HPLC
- The purity of Human B7-H7 is greater than 95% as determined by SEC-HPLC.
Human B7-H7/HHLA2 Protein ELISA Data
- Immobilized Human CD28H, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
Human B7-H7/HHLA2 Protein SPR Data
- Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay (Biacore T200).
