Description
Accession
O75144
Source
DNA sequence encoding Human B7-H2/ICOSLG including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human B7-H2/ICOSLG is a protein consisting of amino acid residues [Asp19-Ser258] ,The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-H2/ICOSLG was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Methods
Human B7-H2/ICOSLG Protein Tris-Bis PAGE
- Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human B7-H2/ICOSLG Protein SEC-HPLC
- The purity of Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Human B7-H2/ICOSLG Protein ELISA Data
- Immobilized Human B7-H2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA.
Human B7-H2/ICOSLG Protein SPR Data
- Human ICOS (Q50A) , hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200).
