Human APOE4/Apolipoprotein E Protein Recombinant
Category: Receptors$193.00 – $641.00
Description
Accession
AAB59397
Source
DNA sequence encoding Human APOE4/Apolipoprotein E including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human APOE4/Apolipoprotein E is a protein consisting of amino acid residues [Lys19-His317] ,The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human APOE4/Apolipoprotein E was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human APOE4/Apolipoprotein E Protein Tris-Bis PAGE
- Human APOE4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human APOE4/Apolipoprotein E Protein SPR Data
- Human LILRB4, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE 4, His Tag with an affinity constant of 74.20 nM as determined in SPR assay (Biacore T200).
