Description
Accession
Q13740
Source
DNA sequence encoding Human ALCAM/CD166 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human ALCAM/CD166 is a protein consisting of amino acid residues [Trp28-Ala526] ,The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human ALCAM/CD166 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Methods
Human ALCAM/CD166 Protein Tris-Bis PAGE
- Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human ALCAM/CD166 Protein SEC-HPLC
- The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Human ALCAM/CD166 Protein ELISA Data
- Immobilized Human ALCAM, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA.
Human ALCAM/CD166 Protein SPR Data
- Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 μM as determined in SPR assay (Biacore T200).
