Description
Accession
Q9BYF1
Source
DNA sequence encoding Human ACE2/ACEH including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human ACE2/ACEH is a protein consisting of amino acid residues [Gln18-Ser740] ,The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human ACE2/ACEH was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q6Q1S2
Biological Process
Molecular function
Molecular function
Methods
Human ACE2/ACEH Protein Tris-Bis PAGE
- Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human ACE2/ACEH Protein SEC-HPLC
- The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC
Human ACE2/ACEH Protein ELISA Data
- Immobilized Human ACE2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 24.5ng/ml determined by ELISA.
Human ACE2/ACEH Protein SPR Data
- SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
