Description
Accession
Q6ISS4
Source
DNA sequence encoding Huma LAIR2/CD306 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Huma LAIR2/CD306 is a protein consisting of amino acid residues [Gln22-Pro152] ,The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Huma LAIR2/CD306 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Huma LAIR2/CD306 Protein Tris-Bis PAGE
- Huma LAIR2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Huma LAIR2/CD306 Protein SEC-HPLC
- The purity of Huma LAIR2 is greater than 95% as determined by SEC-HPLC.
Huma LAIR2/CD306 Protein SPR Data
- Human LAIR2, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.95 nM as determined in SPR assay (Biacore T200).
