Cynomolgus TNFR1/CD120a/TNFRSF1A Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
A0A7N9CVF2
Source
DNA sequence encoding Cynomolgus TNFR1/CD120a/TNFRSF1A including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A is a protein consisting of amino acid residues [Leu30-Thr211] ,The protein has a predicted MW of 21.62 kDa. Due to glycosylation, the protein migrates to 32-42 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus TNFR1/CD120a/TNFRSF1A was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus TNFR1/CD120a/TNFRSF1A Protein Tris-Bis PAGE
- Cynomolgus TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus TNFR1/CD120a/TNFRSF1A Protein SEC-HPLC
- The purity of Cynomolgus TNFR1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus TNFR1/CD120a/TNFRSF1A Protein SPR Data
- Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
