Description
Accession
P79337
Source
DNA sequence encoding Cynomolgus TNF alpha including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus TNF alpha is a protein consisting of amino acid residues [Val77-Leu233] ,The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus TNF alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Cynomolgus TNF alpha Protein Tris-Bis PAGE
- Cynomolgus TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus TNF alpha Protein SEC-HPLC
- The purity of Cynomolgus TNF alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus TNF alpha Protein ELISA Data
- Immobilized Cynomolgus TNF alpha, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
Cynomolgus TNF alpha Protein SPR Data
- Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
