Cynomolgus SIRP alpha/CD172a Protein Recombinant
Category: Receptors$193.00 – $641.00
Description
Accession
NP_001271679
Source
DNA sequence encoding Cynomolgus SIRP alpha/CD172a including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus SIRP alpha/CD172a is a protein consisting of amino acid residues [Glu31-Arg370] ,The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus SIRP alpha/CD172a was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus SIRP alpha/CD172a Protein Tris-Bis PAGE
- Cynomolgus SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus SIRP alpha/CD172a Protein SEC-HPLC
- The purity of Cynomolgus SIRP alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus SIRP alpha/CD172a Protein ELISA Data
- Immobilized Cynomolgus SIRP alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Cynomolgus SIRP alpha/CD172a Protein SPR Data
- Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
