Description
Accession
A2V9Z4
Source
DNA sequence encoding Cynomolgus Serum Albumin including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus Serum Albumin is a protein consisting of amino acid residues [Asp25-Ala608] ,The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus Serum Albumin was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus Serum Albumin Protein Tris-Bis PAGE
- Cynomolgus Serum Albumin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus Serum Albumin Protein SEC-HPLC
- The purity of Cynomolgus Serum Albumin is greater than 95% as determined by SEC-HPLC.
Cynomolgus Serum Albumin Protein SPR Data
- Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.108μM as determined in SPR assay (Biacore T200).
