Cynomolgus/Rhesus macaque FcRn Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
Q8SPV9
Source
DNA sequence encoding Cynomolgus/Rhesus macaque FcRn including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus/Rhesus macaque FcRn is a protein consisting of amino acid residues [Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)] ,The protein has a predicted MW of 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus/Rhesus macaque FcRn was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus/Rhesus macaque FcRn Protein Tris-Bis PAGE
- Cynomolgus/Rhesus macaque FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus/Rhesus macaque FcRn Protein SEC-HPLC
- The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.
Cynomolgus/Rhesus macaque FcRn Protein SPR Data
- Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-HER2 Antibody with an affinity constant of 0.18 μM as determined in SPR assay (Biacore T200).
Cynomolgus/Rhesus macaque FcRn Protein
- Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.108 μM as determined in SPR assay (Biacore T200).
