Description
Accession
 A0A2K5WVV8
Source
DNA sequence encoding Cynomolgus PVRIG including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus PVRIG is a protein consisting of amino acid residues [Thr41-Asp171] ,The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus PVRIG was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus PVRIG Protein Tris-Bis PAGE
- Cynomolgus PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus PVRIG Protein SEC-HPLC
- The purity of Cynomolgus PVRIG is greater than 95% as determined by SEC-HPLC.
Cynomolgus PVRIG Protein ELISA Data
- Immobilized Cynomolgus PVRIG, hFc Tag at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PVRIG Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Cynomolgus PVRIG Protein SPR Data
- Cynomolgus PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 0.23 μM as determined in SPR assay (Biacore T200).
