Description
Accession
Q68VD2
Source
DNA sequence encoding Cynomolgus NKG2A&CD94 including a C-His-Flag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus NKG2A&CD94 is a protein consisting of amino acid residues [Pro94-Leu233(NKG2A) & Lys32-Ile179(CD94)] ,The protein has a predicted MW of 17.01 kDa(NKG2A)&18.27 kDa(CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus NKG2A&CD94 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus NKG2A&CD94 Protein Tris-Bis PAGE
- Cynomolgus NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus NKG2A&CD94 Protein ELISA Data
- Immobilized Cynomolgus NKG2A&CD94, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 8.7g/ml determined by ELISA.
