Cynomolgus M-CSF R/CSF1R/CD115 Protein Recombinant
Category: Receptors$162.00 – $540.00
Description
Accession
G7P8P1
Source
DNA sequence encoding Cynomolgus M-CSF R/CSF1R/CD115 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus M-CSF R/CSF1R/CD115 is a protein consisting of amino acid residues [Ile99-Pro596] ,The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus M-CSF R/CSF1R/CD115 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus M-CSF R/CSF1R/CD115 Protein Tris-Bis PAGE
- Cynomolgus M-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus M-CSF R/CSF1R/CD115 Protein SEC-HPLC
- The purity of Cynomolgus M-CSF R is greater than 95% as determined by SEC-HPLC.
Cynomolgus M-CSF R/CSF1R/CD115 Protein ELISA Data
- Immobilized Cynomolgus M-CSF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA.
Cynomolgus M-CSF R/CSF1R/CD115 Protein SPR Data
- Cynomolgus M-CSF R, His Tag immobilized on CM5 Chip can bind Cynomolgus M-CSF, His Tag with an affinity constant of 0.889 μM as determined in SPR assay (Biacore T200).
