Cynomolgus LILRB2/CD85d/ILT4 Protein Recombinant
Category: Receptors$220.00 – $708.00
Description
Accession
XM_015441717
Source
DNA sequence encoding Cynomolgus LILRB2/CD85d/ILT4 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus LILRB2/CD85d/ILT4 is a protein consisting of amino acid residues [Gly24-Arg457] ,The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus LILRB2/CD85d/ILT4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus LILRB2/CD85d/ILT4 Protein Tris-Bis PAGE
- Cynomolgus LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus LILRB2/CD85d/ILT4 Protein SEC-HPLC
- The purity of Cynomolgus LILRB2 is greater than 95% as determined by SEC-HPLC.
Cynomolgus LILRB2/CD85d/ILT4 Protein SPR Data
- Cynomolgus LILRB2, His Tag immobilized on CM5 Chip can bind Cynomolgus HLA-G Complex Tetramer, His Tag with an affinity constant of 852 nM as determined in SPR assay (Biacore T200).
