Description
Accession
G7P4V0
Source
DNA sequence encoding Cynomolgus IL-17F including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus IL-17F is a protein consisting of amino acid residues [Arg31-Gln163] ,The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus IL-17F was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus IL-17F Protein Tris-Bis PAGE
- Cynomolgus IL-17F on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus IL-17F Protein SEC-HPLC
- The purity of Cynomolgus IL-17F is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-17F Protein ELISA Data
- Immobilized Cynomolgus IL-17F, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-17R alpha, hFc Tag with the EC50 of 2.4μg/ml determined by ELISA.
Cynomolgus IL-17F Protein SPR Data
- Human IL-17R alpha, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-17F, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).
