Description
Accession
XP_005552816
Source
DNA sequence encoding Cynomolgus IL-17A/CTLA-8 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus IL-17A/CTLA-8 is a protein consisting of amino acid residues [Gly24-Ala155] ,The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 26-32 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus IL-17A/CTLA-8 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus IL-17A/CTLA-8 Protein Tris-Bis PAGE
- Cynomolgus IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus IL-17A/CTLA-8 Protein SEC-HPLC
- The purity of Cynomolgus IL-17A is greater than 92% as determined by SEC-HPLC.
Cynomolgus IL-17A/CTLA-8 Protein ELISA Data
- Immobilized Cynomolgus IL-17A, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 31.4ng/ml determined by ELISA.
