Description
Accession
Q0PW92
Source
DNA sequence encoding Cynomolgus IL-13 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Cynomolgus IL-13 is a protein consisting of amino acid residues [Ser19-Asn132] ,The protein has a predicted MW of 13.59 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus IL-13 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Cynomolgus IL-13 Protein Tris-Bis PAGE
- Cynomolgus IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Cynomolgus IL-13 Protein SEC-HPLC
- The purity of Cynomolgus IL-13 is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-13 Protein SPR Data
- Cynomolgus IL-13Ra1,His Tag immobilized on CM5 Chip can bind Cynomolgus IL-13, His Tag with an affinity constant of 21.16 nM as determined in SPR assay (Biacore T200).
